Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Amrit Misra
Date created: 2008-10-11 11:00:00
Date modified: 2015-05-08 01:10:13
nifHp driving mutant NifL in Leucine Landing Pad
| Types | DnaRegion |
| Roles | Plasmid plasmid |
| Sequences | BBa_K138001_sequence (Version 1) |
Description
BBa_K138000 was cut with NdeI/SpeI and ligated with a mutant form of NifL digested with NdeI/SpeI. The mutant form of NifL binds and sequesters NifA with a higher affinity than the wild-type NifL does.Notes
NifA and NifL are proteins involved in nitrogen fixation in Azobacter vinelandii.Source
BBa_K138000 was created by the Michigan Team for iGEM 2008. NifL was a PCR product from pIM18. We received pIM18 form Dr. Ray Dixon, of the John Innes' Center in the United Kingdom| Sequence Annotation | Location | Component / Role(s) |
| BBa_K138000 | 1,284 | engineered_region feature/BioBrick |