BBa_K1381024

BBa_K1381024 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1381024
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Viktor Blomkvist, Tim Hagelby Edstrom, Laura Pacoste and Tersea Reinli
Date created: 2014-10-06 11:00:00
Date modified: 2015-05-08 01:10:13

B0034-PgsA-(EAAAR)x4



    • Details

    Types
    DnaRegion

    Roles
    tag

    Tag

    Sequences BBa_K1381024_sequence (Version 1)

    Description

    Anchor tag to make fusion protein for cell surface display

    Notes

    Designed with freiburg fusion standard but ony got it to work by using normal E, X, S, P restriction

    Source

    PgsA gene from Bacillus Subtilis

    Sequence Annotation Location Component / Role(s)
    B0034
    PgsA
    TACTAG
    (EAAAR)x4
    AgeI-site
    TAA stop
    		1,12
    19,1158
    13,18
    1159,1218
    1219,1224
    1225,1227
    		feature/rbs ribosome_entry_site
    tag feature/tag
    feature/scar
    feature/misc sequence_feature
    feature/misc sequence_feature
    stop_codon feature/stop

    igem#experience
    None
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1381024/1
     

    Attachments

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