BBa_K1392910

BBa_K1392910 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1392910

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Dorukcan ??zk??se

Date created: 2014-10-09 11:00:00

Date modified: 2015-05-08 01:10:15

with L+ arabinose promoter, caterchol 2,3-dioxygenase activate to degration of catechol.

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1392910_sequence (Version 1)

Description

Catechol or catechol 2,3-dioxygenases (XylE) (C2,3O) + O(2) is converted by a ring cleavage into 2-hydroxymuconate semialdehyde which is the cytotoxic and bright yellow-coloured product[1]. This is a key enzyme in many (soil) bacterial species used for the degradation of aromatic compounds. Catechol 2,3-dioxygenase[2] was originally isolated from Pseudomonas putida and is active only as a homotetramer. With araC-Pbad promoter,the production of XylE start and bacteria degrade catechol to 2-hydroxymuconate semialdehyde.

Notes

BBa_K808000 + BBa_K316003

Source

BBa_K808000 + BBa_K316003

Components

Access	Instance	Definition
public	BBa_K808000	Ara
public	BBa_K316003	BBa_K316003

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K808000	1,1209	Ara
BBa_K316003	1218,2278	BBa_K316003

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1392910/1

Member of these Collections

• iGEM Parts Registry