Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Şeniz Y??ksel İlkem Kumru
Date created: 2014-10-09 11:00:00
Date modified: 2015-05-08 01:10:15
Holin+Terminator
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1392971_sequence (Version 1) |
Description
This part comes from a Bacteriophage Holin Gene pS105This is a variation of the S gene of the "Cell Lysis Cassette." When it is paired with an activator, the cassette is expressed and cell lysis is induced.
The S105 gene has the initial 6 base pairs at the beginning of the wild type S gene sequence removed. This causes cell lysis to be induced at a faster rate than the wild type.
The added Terminator is artificial and large.
Notes
No design considerations during Holin GeneBidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 9 residues.
Source
Brown iGEM Team by Ry Young at Texas A&MModified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
| Access | Instance | Definition |
| public public | BBa_K124014 BBa_B1006 | BBa_K124014 BBa_B1006 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K124014 BBa_B1006 | 1,317 326,364 | BBa_K124014 BBa_B1006 |