Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Şeniz Y??ksel
Date created: 2014-10-09 11:00:00
Date modified: 2015-05-08 01:10:15
AracC+Antiholin+RBS+TetLVA+Term+TetR Promoter+ T4 Endolysin+Holin+Terminator
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1392973_sequence (Version 1) |
Description
This part is a long Composite which contains many parts.Holin part comes from a Bacteriophage Holin Gene pS105 This is a variation of the S gene of the "Cell Lysis Cassette." When it is paired with an activator, the cassette is expressed and cell lysis is induced. The S105 gene has the initial 6 base pairs at the beginning of the wild type S gene sequence removed. This causes cell lysis to be induced at a faster rate than the wild type. The added Terminator is artificial and large.
Composite part fot TetLVA TetPromoter and T4:
This part is a composite structure which contains a promoter, RBS side and and a tetracycline repressor from transposon Tn10. It is transcribed in the opposite direction for the coding sequence of the repressor AraC. Through the binding to L+arabinose, the conformation of AraC changes.This causes the protein to diffuses from the DNA thereby inducing transcription. Through the RBS, the definition of the RBS efficiency could be observed. As the second part we added Terminator+Tetr Promoter+T4 Endolysin. For this part an artifical Terminator is used (generally added to another parts). The promoter is a TetR repressible promoter.It is sequenced for pTet inverting regulator. Promoter is constitutively ON and repressed by TetR. TetR repression is inhibited by the addition of tetracycline or its analog part
Notes
No design considerations during Holin GeneBidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 9 residues.
L(+) - Arabinose is in sugar form and harmless The designed promoter has low background activity, so the membrane proteins will not damage the cells before induction. Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 9 residues. High expression of lysozyme only is toxic to bacteria. BBa_R0040 (TetR repressible promoter) is based on a cI promoter. It has been modified to include two TetR binding sites. Constructed by DNA synthesis.
Source
Brown iGEM Team by Ry Young at Texas A&M Modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairsReferences: //parts.igem Schleif, R. (2000). "Regulation of the L-arabinose operon of Escherichia coli." Trends Genet 16(12): 559-565. Ren, H., D. Yu, et al. (2009). "High-level production, solubilization and purification of synthetic human GPCR chemokine receptors CCR5, CCR3, CXCR4 and CX3CR1 http://openwetware.org/wiki/Titratable_control_of_pBAD_and_lac_promoters_in_individual_E._coli_cells#pBAD_promotersOpenWetWare http://www.ncbi.nlm.nih.gov/pubmed/7768852?dopt=Abstract Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=66503&to=66997&view=gbwithparts modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs BiblioPlus Extension Error fetching PMID 9092630:
| Access | Instance | Definition |
| public public public public public public public public | BBa_K808000 BBa_K515004 BBa_C0040 BBa_B1006 BBa_R0040 BBa_K112806 BBa_K124014 BBa_B1006 | Ara BBa_K515004 tetR BBa_B1006 p(tetR) BBa_K112806 BBa_K124014 BBa_B1006 |