Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dorukcan ??zk??se
Date created: 2014-10-09 11:00:00
Date modified: 2015-05-08 01:10:15
with constitutive promoter (J23108), XylE(K316003) coding sequence
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1392991_sequence (Version 1) |
Description
Parts J23100 through J23119 are a family of constitutive promoter parts isolated from a small combinatorial library. J23119 is the "consensus" promoter sequence and the strongest member of the family. All parts except J23119 are present in plasmid J61002. Part J23119 is present in pSB1A2.Catechol or catechol 2,3-dioxygenases (XylE) (C2,3O) + O(2) is converted by a ring cleavage into 2-hydroxymuconate semialdehyde which is the cytotoxic and bright yellow-coloured product[1]. This is a key enzyme in many (soil) bacterial species used for the degradation of aromatic compounds. Catechol 2,3-dioxygenase[2] was originally isolated from Pseudomonas putida and is active only as a homotetramer. With araC-Pbad promoter,the production of XylE start and bacteria degrade catechol to 2-hydroxymuconate semialdehyde.Notes
BBa_J23108 BBa_K1392910Source
BBa_J23108 BBa_K1392910| Access | Instance | Definition |
| public public | BBa_J23108 BBa_K316003 | BBa_J23108 BBa_K316003 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23108 BBa_K316003 | 1,35 44,1104 | BBa_J23108 BBa_K316003 |