BBa_K1393001

BBa_K1393001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1393001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jiajun Tan
Date created: 2014-10-01 11:00:00
Date modified: 2015-07-22 02:48:08

OprF(Ala196)+CBP



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1393001_sequence (Version 1)

Description

The OprF is a major outer membrane protein of Pseudomonas aeruginosa. This protein functions as a nonspecific porin to allow the passage of small hydrophilic molecules, plays a structural role in maintaining cell shape and outer membrane integrity, and is required for growth under low osmolality. The structure of OprF has been proposed to consist of three domains, the N-terminal forming h-barrel structure, a loop or hinge region, and the C-terminal associated with peptidoglycan. Based on the predicted secondary structure and information found in the literature, we chose Ala196 as potential fusion sites for displaying CBP. CBP is the abbreviation of copper binding peptide made up of seven amino acid.

Notes

Based on the predicted secondary structure and information found in the literature, we chose Val(188) as potential fusion sites for displaying CBP. CBP is the abbreviation of copper binding peptide made up of seven amino acid.

Source

The OprF gene is from Pseudomonas aeruginosa.

Sequence Annotation Location Component / Role(s)
oprF to Ala196
CBP
1,588
589,609
CDS feature/protein
feature/binding non_covalent_binding_site
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1393001/1