Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sean Lowe
Date created: 2014-10-04 11:00:00
Date modified: 2016-02-03 10:54:34
Linear Magainin 1 peptide
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1394001_sequence (Version 1) |
Description
This partcodes for their Magainin 1 antimicrobial peptide. It includes a SUMO fusion partner to allow soluble, non-toxic expression of the gene in E. coli.Notes
Because Magainin 1 is normally cytotoxic to E. coli and other bacteria, the SUMO fusion partner is critical to successful expression of the construct. Without SUMO, the antimicrobial peptide would likely lyse the cell membrane.Source
The sequence for Magainin 1 was generated by reverse translating the amino acid sequence for the peptide and then codon optimising it. This gene was then synthesised. The SUMO protein fusion partner was derived from the SUMO biobrick introduced by TU Delft 2013.| Sequence Annotation | Location | Component / Role(s) |
| BBa_K525998 B0034 T7 promoter RBS BBa_K731721 HIS-tagged SUMO Protein Magainin I Antimicrobial Peptide T7 terminator | 1,32 21,32 1,20 24,27 420,467 33,344 345,419 420,467 | feature/BioBrick engineered_region engineered_region feature/BioBrick feature/promoter promoter feature/rbs ribosome_entry_site feature/BioBrick engineered_region CDS feature/protein CDS feature/protein feature/misc sequence_feature |