BBa_K1394002

BBa_K1394002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1394002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sean Lowe
Date created: 2014-10-04 11:00:00
Date modified: 2015-05-08 01:10:15

MAP star peptide



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1394002_sequence (Version 1)

Description

This part codes for a novel star peptide design by the 2013 University of Melbourne iGEM team. The peptide was designed in such a way that it can be functionalised using chemical techniques with various biomacromolecules.

Notes

The protein was designed to both be highly soluble and lacking sequences which would make it prone to proteolytic degradation.

Source

The peptide is completely synthetic and was designed by the University of Melbourne iGEM team. It is expressed with the fusion partner, SUMO, to improve solubility. The SUMO protein sequence was derived from the work of TU Delft 2013 iGEM. The DNA sequence for this protein was codon optimised for E. coli.

Sequence Annotation Location Component / Role(s)
BBa_K525998
T7 promoter
B0034
RBS
HIS-tagged SUMO Protein
T7 terminator
BBa_K731721
USP protein
1,32
1,32
21,32
24,27
33,344
733,812
733,812
345,732
feature/BioBrick engineered_region
feature/promoter promoter
feature/BioBrick engineered_region
ribosome_entry_site feature/rbs
CDS feature/protein
sequence_feature feature/misc
feature/BioBrick engineered_region
CDS feature/protein
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1394002/1