BBa_K1398002

BBa_K1398002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1398002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Exeter iGEM 2014

Date created: 2014-08-10 11:00:00

Date modified: 2015-05-08 01:10:15

NemA (N-ethylmaleimide reductase)

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1398002_sequence (Version 1)

Description

N-ethylmaleimide reductase, created for use as a Trinitroltoluene and Nitroglycerine degrading protein.
NemA is a flavoprotein that primarily catalyses the reduction of N-ethylmaleimide (NEM), which is toxic to cell growth. However, it is also involved in the degradation of other toxic compounds for their reuse in nitrogen metabolism. Some of these compounds include PETN, quinones and chromate. It increases the resistance of organisms to the toxic effects of these compounds.
This sequence only encodes for the protein, an attached His (x6) Tag to allow for purification and a double-STOP codon. It therefore requires regulatory sequences (promoter/RBS/terminator) to be added for expression.
The protein has been codon-optimised for expression in E. coli.

Notes

The original sequence of this part was found using the NCBI database and taken from E. coli. The sequence was then codon-optimised to ensure increased expression. This has resulted in a novel synthetic sequence.

Source

Source of original sequence: Escherichia coli O104:H4 str. C227-11

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1398002/1

Member of these Collections

• iGEM Parts Registry