Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Exeter iGEM 2014
Date created: 2014-08-11 11:00:00
Date modified: 2015-05-08 01:10:15
NemR Intergenic Reporter
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1398004_sequence (Version 1) |
Description
A construct created to test the effectiveness of the NemR upstream intergenic region (BBa_1398005).The construct contains the entire NemR upstream region (BBa_1398005), which contains a promoter and RBS. It is followed by the fluorescent reporter iLOV (listed in the repository as BBa_K660004), a double STOP codon and a double terminator (B0015).
Notes
iLOV was used as a reporter in this construct as it ???outperforms??? GFP.Source
The origin of this region is Escherichia coli JD22799. The regulatory sequences were acquired from the iGEM repository.| Access | Instance | Definition |
| public public public | BBa_K1398005 BBa_K660004 BBa_B0015 | NemR (UIR) BBa_K660004 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1398005 BBa_K660004 BBa_B0015 | 1,284 291,626 635,763 | NemR (UIR) BBa_K660004 BBa_B0015 |