NemR (UIR)

BBa_K1398005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1398005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Exeter iGEM 2014

Date created: 2014-08-11 11:00:00

Date modified: 2015-05-08 01:10:15

NemR Upstream Intergenic Region

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K1398005_sequence (Version 1)

Description

This sequence is found upstream of several NemR regulated genes. It was created for use as a TNT-detection mechanism.
It contains the bases from X+ to the start codon, which include a promoter and RBS.
This region should allow regulation of gene preoducts through the detection of TNT. If it is not present NemR will bind to a specified sequence of bases and inhibit transcription. If TNT is not present NemR will not bind and transcription will complete.

Notes

When studied using bioinformatic databases, such as BLAST, the start codon is incorrectly labelled at position X+. The start codon is correctly positioned at the end of this sequence.

Source

The origin of this region is Escherichia coli JD22799.

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1398005/1

Member of these Collections

• iGEM Parts Registry