Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Exeter iGEM 2014
Date created: 2014-08-11 11:00:00
Date modified: 2015-05-08 01:10:15
NemR Promoter Reporter
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1398007_sequence (Version 1) |
Description
A construct created to test the effectiveness of the NemR recognizing promoter (BBa_1398008).The construct begins with the synthetic promoter NemR, which combines a high-expression promoter with the NemR recognition box (also found in BBa_1398005).
It is followed by a strong RBS (BBa_B0034), the fluorescent reporter iLOV (listed in the repository as BBa_K660004), a double STOP codon and a double terminator (BBa_B0015).
Notes
The promoter used in this construct was synthetically designed and does not exist it nature.iLOV was used as a reporter in this construct as it ???outperforms??? GFP.
Source
The origin of the NemR box is Escherichia coli JD22799. The regulatory sequences were acquired from the iGEM repository.| Access | Instance | Definition |
| public public public public | BBa_K1398008 BBa_B0034 BBa_K660004 BBa_B0015 | NemR (RP) BBa_B0034 BBa_K660004 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1398008 BBa_B0034 BBa_K660004 BBa_B0015 | 1,34 43,54 61,396 405,533 | NemR (RP) BBa_B0034 BBa_K660004 BBa_B0015 |