Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anna Stikane
Date created: 2014-09-18 11:00:00
Date modified: 2015-05-08 01:10:16
Plac-RFP(LVA)
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K1399011_sequence (Version 1) |
Description
Lactose/IPTG inducible promoter with RBS, RFP reporter tagged with LVA-ssrA degradation tag and terminator.The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity.
This part can be considered as improved version of BBa_J04451 as it does not contain StuI restriction site coding for additional 'RP' residues between RFP and LVA tag.
Notes
Part was assembled using BrickClip assembly (BBF RFC 104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]Source
other biobricks| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0010 end of LacI coding region (inactive) CAP binding site -35 -10 LacI binding site start BBa_B0034 conserved start BBa_K1399001 BBa_E1010 w/o stop LVA-ssrA tag stop stop Barcode of BBa_E1010 BBa_B0015 BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop | 1,200 1,88 89,126 137,142 161,166 166,200 173,173 209,220 213,216 227,229 227,940 230,901 902,934 935,937 938,940 941,965 974,1102 974,1053 985,1028 1062,1102 1069,1088 1089,1102 1095,1095 | feature/BioBrick engineered_region feature/cds CDS feature/binding non_covalent_binding_site promoter feature/promoter promoter feature/promoter non_covalent_binding_site feature/binding feature/start start_codon feature/BioBrick engineered_region feature/conserved conserved_region start_codon feature/start feature/BioBrick engineered_region CDS feature/cds tag feature/tag stop_codon feature/stop stop_codon feature/stop feature/misc sequence_feature feature/BioBrick engineered_region feature/BioBrick engineered_region feature/stem_loop stem_loop engineered_region feature/BioBrick feature/stem_loop stem_loop polyA_site feature/polya stop_codon feature/stop |