BBa_K1399016

BBa_K1399016 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1399016

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Anna Stikane

Date created: 2014-09-18 11:00:00

Date modified: 2015-05-08 01:10:16

Plac-GFP(LAA)

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K1399016_sequence (Version 1)

Description

Lactose/IPTG inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator.
The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.

Notes

Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]

Source

other biobricks

Components

Access	Instance	Definition
public	BBa_R0010	LacI
public	BBa_B0034	BBa_B0034
public	BBa_K1399006	BBa_K1399006
public	BBa_B0015	BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010	1,200	LacI
BBa_B0034	209,220	BBa_B0034
BBa_K1399006	227,979	BBa_K1399006
BBa_B0015	988,1116	BBa_B0015

Other Properties

• igem#experience: Works
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1399016/1

Member of these Collections

• //cds/reporter/gfp
• //classic/generator/prct
• //classic/reporter/pret
• //function/reporter/fluorescence
• iGEM Parts Registry