BBa_K1399017

BBa_K1399017 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1399017
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anna Stikane
Date created: 2014-09-18 11:00:00
Date modified: 2015-05-08 01:10:16

Plac-GFP(DAS+2)



Types
DnaRegion

Roles
engineered_region

Reporter

Sequences BBa_K1399017_sequence (Version 1)

Description

Lactose/IPTG inducible promoter with GFP reporter tagged with NYADAS-ssrA degradation tag followed by terminator.
The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.

Notes

Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]

Source

other biobricks

igem#experience
Works
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1399017/1