BBa_K1399017

BBa_K1399017 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1399017

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Anna Stikane

Date created: 2014-09-18 11:00:00

Date modified: 2015-05-08 01:10:16

Plac-GFP(DAS+2)

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K1399017_sequence (Version 1)

Description

Lactose/IPTG inducible promoter with GFP reporter tagged with NYADAS-ssrA degradation tag followed by terminator.
The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.

Notes

Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]

Source

other biobricks

Components

Access	Instance	Definition
public	BBa_R0010	LacI
public	BBa_B0034	BBa_B0034
public	BBa_K1399007	BBa_K1399007
public	BBa_B0015	BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010	1,200	LacI
BBa_B0034	209,220	BBa_B0034
BBa_K1399007	227,985	BBa_K1399007
BBa_B0015	994,1122	BBa_B0015

Other Properties

• igem#experience: Works
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1399017/1

Member of these Collections

• //cds/reporter/gfp
• //classic/generator/prct
• //classic/reporter/pret
• //function/reporter/fluorescence
• iGEM Parts Registry