BBa_K1399022

BBa_K1399022 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1399022

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Anna Stikane

Date created: 2014-09-18 11:00:00

Date modified: 2015-05-08 01:10:16

Ptet-GFP(DAS+2)

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K1399022_sequence (Version 1)

Description

Tetracycline/aTc inducible promoter with GFP reporter tagged with NYADAS-ssrA degradation tag followed by terminator.
The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.

Notes

Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]

Source

other parts

Components

Access	Instance	Definition
public	BBa_R0040	p(tetR)
public	BBa_B0034	BBa_B0034
public	BBa_K1399007	BBa_K1399007
public	BBa_B0015	BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0040	1,54	p(tetR)
BBa_B0034	63,74	BBa_B0034
BBa_K1399007	81,839	BBa_K1399007
BBa_B0015	848,976	BBa_B0015

Other Properties

• igem#experience: None
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1399022/1

Member of these Collections

• //cds/reporter/gfp
• //classic/generator/prct
• //classic/reporter/pret
• //function/reporter/fluorescence
• iGEM Parts Registry