BBa_K1401007

BBa_K1401007 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1401007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kathleen Lewis

Date created: 2014-10-08 11:00:00

Date modified: 2015-05-08 01:10:16

Tandem promoter pTet-pBad

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K1401007_sequence (Version 1)

Description

This tandem promoter was created using the MoClo assembly method. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. This transcriptional unit has Kanamycin resistance. The promoter is induced by atc and arabinose.

The characterization data will be updated before the jamboree.

Notes

We looked through the literature and decided to attach the two promoters without any extra sequence between them

Source

This was formed using BBa_R0040 and BBa_I13453

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1401007/1

Member of these Collections

• iGEM Parts Registry