Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yash Agarwal
Date created: 2014-10-10 11:00:00
Date modified: 2015-05-08 01:10:16
pTet - BCD2 - RFP
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K1401611_sequence (Version 1) |
Description
The parts contain the BioBricks - R0040_FB, BCD2_BC, E1010m_CD and B0015_DF. All of these are MoClo variants i.e. they have MoClo flanking regions attached to each basic part.Notes
All basic BioBricks used were transformed into MoClo variants by adding the following fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. These were then put together using the digestion-ligation MoClo reaction. RFP Reporter (E1010m) has a fixed illegal cut site.Source
E. Coli| Access | Instance | Definition |
| public public public public | BBa_K1114027 BBa_K1114107 BBa_K1114211 BBa_K1114301 | BBa_K1114027 BBa_K1114107 BBa_K1114211 BBa_K1114301 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1114027 BBa_K1114107 BBa_K1114211 BBa_K1114301 | 1,62 71,162 171,859 868,1004 | BBa_K1114027 BBa_K1114107 BBa_K1114211 BBa_K1114301 |