BBa_K1401613

BBa_K1401613 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1401613

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Yash Agarwal

Date created: 2014-10-10 11:00:00

Date modified: 2015-05-08 01:10:16

pLac - BCD2 - araC - YFP

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K1401613_sequence (Version 1)

Description

The parts contain the BioBricks - R0010_EB, BCD2_BC, C0080_CI, E0030_ID and B0015_DF. All of these are MoClo variants i.e. they have MoClo flanking regions attached to each basic part. A new fusion site (I) was specifically designed to create the fusion protein (regulator + reporter).

Notes

All basic BioBricks used were transformed into MoClo variants by adding the following fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA; I = TCTAGA. These were then put together using the digestion-ligation MoClo reaction.

Source

E. Coli

Components

Access	Instance	Definition
public	BBa_K783046	BBa_K783046
public	BBa_K1114107	BBa_K1114107
public	BBa_K1114204	BBa_K1114204
public	BBa_K1114208	BBa_K1114208
public	BBa_K783020	BBa_K783020

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K783046	1,200	BBa_K783046
BBa_K1114107	209,300	BBa_K1114107
BBa_K1114204	309,1231	BBa_K1114204
BBa_K1114208	1240,1970	BBa_K1114208
BBa_K783020	1979,2107	BBa_K783020

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1401613/1

Member of these Collections

• iGEM Parts Registry