Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chen Xi
Date created: 2014-09-28 11:00:00
Date modified: 2015-05-08 01:10:16
Lac-RBS-AtFatA-Treminator
| Types | DnaRegion |
| Roles | sequence_feature Other |
| Sequences | BBa_K1402007_sequence (Version 1) |
Description
Acyl-acyl carrier protein (ACP) thioesterase catalyzes the hydrolysis of acyl-ACP to fatty acids and ACP this reaction removes the coupling between fatty acid biosynthesis and phospholipid biosyntheis,which is a key to obtain free fatty acids.In before study, thioesterase gene AtFatA can express in E.coli BL21(DE3),and increase free fatty acids.This thioesterase can be expressed in the presence of lactose or IPTG.
The RBS and lac promoter are in front of the AtFatA coding sequence and T7 terminator is after AtFatA. We can activate the expression of AtFatA by adding lactose in the culture in the absence of glucose.You can only use this part in E.coli BL21(DE3) because the lac promoter we use is the hybrid of lac promoter and T7 promoter.
Notes
noSource
Arabidopsis thaliana| Access | Instance | Definition |
| public public | BBa_K1402005 BBa_K1402010 | BBa_K1402005 BBa_K1402010 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1402005 BBa_K1402010 | 1,1558 1567,1645 | BBa_K1402005 BBa_K1402010 |