Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sierra Tackett
Date created: 2014-10-08 11:00:00
Date modified: 2015-05-08 01:10:17
pLAC promoter + strong RBS
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1406001_sequence (Version 1) |
Description
A combinatorial part of R0011, B0034, and a NheI cutsite. This allows a in-frame, orientated insertion of a biobrick part behind the pLac promoter and the strong RBS using the restriction enzymes NheI and PstI.Notes
We were hoping to make a high-expressing, directional insertion system in which we used enzymes which were compatible with the BioBrick standard and as an end product form a BioBrick.Source
phage lamda| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0011 lac O1 -35 lac O1 -10 conserved BBa_B0034 | 1,54 3,19 20,25 26,42 43,48 68,71 64,75 | engineered_region feature/BioBrick feature/operator operator feature/promoter promoter feature/operator operator feature/promoter promoter conserved_region feature/conserved feature/BioBrick engineered_region |