BBa_K1408000

BBa_K1408000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1408000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stephen A Rettie
Date created: 2014-10-08 11:00:00
Date modified: 2015-05-08 01:10:18

Degron (unstable protein domain)



Types
DnaRegion

Roles
Protein_Domain

polypeptide_domain

Sequences BBa_K1408000_sequence (Version 1)

Description

This protein domain when fused to a protein acts as a source of instability leading to degradation by the cell via ubiquitination.

Notes

Xbe1 and Spe1 restriction sites were in the original sequence obtained from the Fields Lab. The submitted part was synthesized with synonymous substitutions (silent mutations) amplified with primers containing the biobrick prefix and suffix and then digested and ligated into the iGEM vector.

Source

Protein domain Saccharomyces cerevisiae MATa2 (a=alpha). This part was obtained from the Fields Lab at the University of Washington.

Sequence Annotation Location Component / Role(s)
Start Codon
Protein
cds
1,3
1,204
1,204
feature/start start_codon
CDS feature/protein
feature/cds CDS
igem#experience
None
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1408000/1