BBa_K1413021

BBa_K1413021 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1413021

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Laura MATABISHI, Julie Zaworski

Date created: 2014-10-06 11:00:00

Date modified: 2015-05-08 01:10:18

bphR2 mutated

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1413021_sequence (Version 1)

Description

bphR2 gene, from Pseudomonas pseudoalcaligenes KF707, encodes for a regulatory protein (bphR2) which can detect polychlorinated biphenyls (PCBs). This gene regulates negatively bphR1 promoter involved in the transcription of biphenyl-catabolic genes (bph), degrading PCBs.
In absence of PCB, bphR2 is bound to the bphR1 promoter. When compound diffuses into the cell, it binds to protein allowing a conformational change. BphR2 is release from the promoter that its allows the transcription of bph genes and therefore the degration of PCBs.

Our aim was to finish and characterize the part of 2013 Paris Saclay's team. We used bphR2 in our biosensor project of PCBs. Instead of the genes of degradation, we have RFP (BBa_E1010) to detect 2 hydroxy-3',4' biphenyl.

Notes

Moreover, the sequence contained a pstI site from 142 to 148bp after ATG site so we have synthetized it by mutating the site, keeping the same amino acid.

Source

The gene was synthetized from the sequence of bphR2 (BBa_K1155009)because there isn't the sample in the registry.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
bphR2	1,903	feature/protein, CDS
mutation	142,147	sequence_alteration, feature/mutation

Other Properties

• igem#experience: None
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1413021/1

Member of these Collections

• iGEM 2018 Distribution Plate 5
• iGEM 2018 Distribution Plate 5 Well 10L
• iGEM 2018 Part Library
• iGEM 2018 Plasmid pSB1C3
• iGEM Parts Registry