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BBa_K1413024

BBa_K1413024 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1413024

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Laura MATABISHI, Nandjafot MENDY

Date created: 2014-10-16 11:00:00

Date modified: 2015-05-08 01:10:18

bphR1-RBS-RFP-Terminator

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Details

Types	DnaRegion
Roles	Composite engineered_region
Sequences	BBa_K1413024_sequence (Version 1)

Description

bphR1 promoter, from Pseudomonas pseudoalcaligenes KF707, activates the bph genes of biphenyls degradation, in presence of bphR2 protein and biphenyl.
In our study, the aim was to detect the presence of polychlorinated biphenyls (PCBs) with RFP fluorescence.

Notes

Between each part, there is the scar SpeI/XbaI

Source

All the parts come from the registry

Components

Access	Instance	Definition
public public public public public public public	BBa_K1155001 BBa_G0001 BBa_B0034 BBa_G0001 BBa_E1010 BBa_G0001 BBa_B0015	BBa_K1155001 scar BBa_B0034 scar mRFP1 scar BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1155001 BBa_G0001 BBa_B0034 BBa_G0001 BBa_E1010 BBa_G0001 BBa_B0015	1,300 301,308 309,320 321,328 329,1034 1035,1042 1043,1171	BBa_K1155001 scar BBa_B0034 scar mRFP1 scar BBa_B0015

Other Properties

igem#experience	None
igem#sampleStatus	Not in stock
igem#status	Unavailable
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K1413024/1