IodoY RS

BBa_K1416001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1416001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jordan Monk

Date created: 2014-10-05 11:00:00

Date modified: 2015-05-08 01:10:20

The tRNA synthetase/tRNA needed for incorporating 3-iodo-L-tyrosine (IodoY) at a UAG codon

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1416001_sequence (Version 1)

Description

The part includes the gene coding for the Methanocaldococcus jannaschii synthetase that charges the orthogonal tRNA (also in the part) with the amino acid tyrosine along with the proper promoters and terminator. This part encodes for the incorporation of tyrosine during translation at AUG amber stop codons in a cell with Release Factor 1 (RF1) knocked out such as "Amberless" E. coli or "RF0".

Source

This part is from the genomic sequence of Methanocaldoccus jannaschii. Wang L, et al. Expanding the Genetic Code of Escherichia coli. Science 20 April 2001: 292 (5516), 498-500.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
IodoYRS promoter	72,219	promoter, feature/promoter
IodoYRS	233,1153	feature/protein, CDS
tRNA promoter	1330,1379	promoter, feature/promoter
tRNA	1396,1471	CDS, feature/cds
terminator	1497,1526	feature/stem_loop, stem_loop

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1416001/1

Member of these Collections

• iGEM Parts Registry