Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mengni Wang
Date created: 2014-10-04 11:00:00
Date modified: 2015-05-08 01:10:22
pRPAI
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K1421005_sequence (Version 1) |
Description
In the photosynthetic bacterium Rhodopseudomonas palustris, there is a quorum-sensing system RPAI/RPAR, which is similar to the LUXI/LUXR system in V. Fischeri. RpaI is the acyl-HSL synthase to produce p-coumaroyl-HSL by using environmental p-coumaric acid. RPAR is a signal receptor with homology to fatty acyl-HSL receptors (such as LuxR) that responds to p-coumaroyl-HSL to regulate global gene expression including the promoter of RPAI, pRPAI.[1]In deed, there have already been numbers of quorum-sensing systems; usually, the signals of these systems are not unique and inter-influence the other. While, p-coumaroyl-HSL (pc-HSL), the signal of RPAI/RPAR system is unique to AHL. So, RPA system is independent on LUX system and a part of its homologies.
This part, pRpaI, is the promoter of RPAI. It opens the expression of the gene behind and meanwhile, can be regulated by the complex RPAR/pc-HSL.
Reference :
[1] 3.quorum sensing system in Rhodopseudomonas palustris:Schaefer A L, Greenberg E P, Oliver C M, et al. A new class of homoserine lactone quorum-sensing signals[J]. Nature, 2008, 454(7204): 595-599.
Notes
This part???s sequence comes from the genome of Rhodopseudomonas palustris, and we did two nucleotides??? modification to make it match the requirements of partand then synthesized it.Source
This part???s sequence comes from the genome of Rhodopseudomonas palustris, and we did two nucleotides??? modification to make it match the requirements of part and then synthesized it.| Sequence Annotation | Location | Component / Role(s) |
| lux box-like elements 1 lux box-like elements 2 | 1,20 43,63 | non_covalent_binding_site feature/binding feature/binding non_covalent_binding_site |