Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mengni Wang
Date created: 2014-10-04 11:00:00
Date modified: 2015-05-08 01:10:22
pRPAI+RPAI
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K1421007_sequence (Version 1) |
Description
In the photosynthetic bacterium Rhodopseudomonas palustris, there is a quorum-sensing system RPAI/RPAR, which is similar to the LUXI/LUXR system in V. Fischeri. RpaI is the acyl-HSL synthase to produce p-coumaroyl-HSL by using environmental p-coumaric acid. RPAR is a signal receptor with homology to fatty acyl-HSL receptors (such as LuxR) that responds to p-coumaroyl-HSL to regulate global gene expression including the promoter of RPAI, pRPAI.[1]In deed, there have already been numbers of quorum-sensing systems; usually, the signals of these systems are not unique and inter-influence the other. While, p-coumaroyl-HSL (pc-HSL), the signal of RPAI/RPAR system is unique to AHL. So, RPA system is independent on LUX system and a part of its homologies.
This part is comprised of promoter of RPAI and coding region of RPAI. To use this device, one should put 1mM p-coumarate in the plate and pc-HSL, the signal of RPA system, will be produced.
Reference :
[1] 3.quorum sensing system in Rhodopseudomonas palustris:Schaefer A L, Greenberg E P, Oliver C M, et al. A new class of homoserine lactone quorum-sensing signals[J]. Nature, 2008, 454(7204): 595-599.