Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Connor McBrine
Date created: 2014-10-16 11:00:00
Date modified: 2015-05-08 01:10:23
merRNA, c-di-GMP aptamer with promoter and terminators
| Types | DnaRegion |
| Roles | mature_transcript_region RNA |
| Sequences | BBa_K1427001_sequence (Version 1) |
Description
This part contains BBa_K1427001 under the transcriptional control of a constitutive T7 promoter. Additionally, terminators developed by the Voigt Lab flank the merRNA sequence. There is an upstream ECK120033737 terminator which stops read-through by other genes. Downstream of the merRNA sequence, bidirectional termination is ensured with ECK120029600 and ECK120033736. It is likely these are redundant, as the poly-U tail of the merRNA suggests that is contains an intrinsic terminator.Notes
The T7 promoter initiates transcription 2 nucleotides (GG) upstream of the actual merRNA sequence. Predictive RNA folding programs did not report any change in secondary structure with this small 5' addition.Source
Bdellovibrio bacteriovorus| Sequence Annotation | Location | Component / Role(s) |
| merRNA ECK120033737 T7 promoter ECK120029600 ECK120033736 | 77,521 1,57 58,76 522,611 612,664 | engineered_region feature/BioBrick sequence_feature feature/misc feature/promoter promoter feature/misc sequence_feature feature/misc sequence_feature |