Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Cedric Bl??tz
Date created: 2014-09-29 11:00:00
Date modified: 2015-05-08 01:10:23
yeast TRP1 gene
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1430003_sequence (Version 1) |
Description
Selective marker for auxotrophic strains of yeast after transforamtion with plasmid that contains this gene.What does the marker help synthesize?
L-tryptphane
Where it can be used?
In TRP1 deficient strains of S. cerevisiae
Notes
We mutated the restriction site for Xba1 in the sequence that is gets compatibel to Prefix/Suffix enzymes. We used PCR approch with primers that add a different base in the recognition siteSource
genomic DNA of S. cerevisiae| Sequence Annotation | Location | Component / Role(s) |
| TRP1 s_mutation | 1,675 87,87 | CDS feature/protein feature/s_mutation point_mutation |