Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: James Chappell
Date created: 2008-09-10 11:00:00
Date modified: 2015-05-08 01:10:23
Promoter veg a constitutive promoter for B. subtilis
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K143012_sequence (Version 1) |
Description
Pveg is a constitutive promoter that constitutively expresses the P43 protein in B.subtilis. Pveg contains binding sites for the B.sutbilis major sigma factor#1. Pveg in B.subtilis utilises two binding sites to cause high expression of genes#2, however our Pveg is lacking the upstream site to give a medium level of gene expression. It has been noted that the sporulation master regulatoion factor spoOA interacts with Pveg though it is not known how#3. The context with which we used the promoter Pveg is as a Polymerase Per Second (PoPS) generator.Notes
The biobrick part was designed to include a single binding site for the B.subtilis major sigma factor. In addition the biobrick standard was applied to the promoter Pveg sequence.Source
The Pveg promoter was suggested to us by Dr. Jan-Willem Veening of Newcastle University. This sequence supplied was compared to that of the DBTBS database#3 then a section containing the binding site synthesised by Geneart.| Sequence Annotation | Location | Component / Role(s) |
| Sigma A-35 Sigma A -10 | 63,68 86,91 | promoter feature/promoter feature/promoter promoter |