Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: James Chappell
Date created: 2008-09-10 11:00:00
Date modified: 2015-05-08 01:10:23
Promoter 43 a constitutive promoter for B. subtilis
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K143013_sequence (Version 1) |
Description
Promoter 43 is a constitutive promoter that constitutively expresses the P43 protein in B.subtilis. This promoter has been shown to be recognized and active during the exponential and lag phases of growth. It has been hypothesized that the ability to recognize the promoter in exponential and lag phase of growth is due to the recognition of the promoter by both sigma factor 55 (the major sigma factor) and sigma factor 37 (the lag phase sigma factor) 1. The P43 promoter has been previously used for constitutive expression of exogenous genes within B.subtilis vectors 2. The context with which we used the promoter P43 is as a Polymerase Per Second (PoPS) generator.Notes
The biobrick part was designed to include the binding sites for both the sigma factor A and B. In addition the biobrick standard was applied to the promoter P43 sequence.Source
The part was designed using the sequence from the B.subtilis genome and from previously published papers 2. This sequence was then synthesised by Geneart.| Sequence Annotation | Location | Component / Role(s) |
| Sigma B -35 Sigma B -10 Sigma A -35 Sigma A -10 | 14,22 38,47 24,29 47,52 | promoter feature/promoter feature/promoter promoter promoter feature/promoter feature/promoter promoter |