Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: James Chappell
Date created: 2008-09-14 11:00:00
Date modified: 2015-05-08 01:10:23
Promoter Xyl for B.subtilis
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K143014_sequence (Version 1) |
Description
Promoter Xylose is an inducible promoter that has been designed for high expression in B.subtilis. Gene expression under the promoter xylose can be induced by addition of xylose. The context with which we used the promoter xylose, was to take an input of xylose and give Polymerase Per Second(PoPS) as an output.Promoter xylose is an inducible promoter that has been designed for high expression in B.subtilis. Gene expression under the promoter xylose can be induced by addition of xylose. The context with which we used the promoter xylose, was to take an input of xylose and give Polymerase Per Second(PoPS) as an output. Xylose does not induce the promoter xylose directly, but requires the transcriptional regulator XylR, (
Notes
Biobrick standard was applied to the promoter xylose sequence.Source
The part was designed using the sequence from the B.subtilis genome and from previously published papers 12. This sequence was then synthesised by Geneart.| Sequence Annotation | Location | Component / Role(s) |
| XylR Operator XylR Operator Sigma A -35 Sigma A -10 | 51,61 65,75 13,18 36,41 | operator feature/operator operator feature/operator feature/promoter promoter promoter feature/promoter |