Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chris Hirst
Date created: 2008-09-17 11:00:00
Date modified: 2015-05-08 01:10:23
Promoter hyper-spank for B. subtilis
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K143015_sequence (Version 1) |
Description
Promoter hyper-spank is an inducible promoter that has been designed for high expression in B.subtilis. Gene expression under the promoter hyper-spank can be induced by addition of Isopropyl β-D-1-thiogalactopyranoside (IPTG). The context with which we used the promoter hyper-spank, was to take an input of IPTG and give Polymerase Per Second(PoPS) as an output. IPTG does not induce the promoter hyper-spank directly, but requires the transcriptional regulator LacI, (Notes
Biobrick standard was applied to the promoter hyper-spank sequence.Source
The part was designed using the sequence from the B.subtilis genome and from previously published papers 123. This sequence was then synthesised by Geneart.| Sequence Annotation | Location | Component / Role(s) |
| LacI Operator Sigma A -35 Sigma A -10 LacI Operator | 10,30 46,50 69,74 81,101 | operator feature/operator feature/promoter promoter promoter feature/promoter feature/operator operator |