EpsE

BBa_K143032 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K143032

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Chris Hirst

Date created: 2008-09-17 11:00:00

Date modified: 2015-05-08 01:10:24

EpsE Molecular Clutch Gene of B. subtilis

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K143032_sequence (Version 1)

Description

The epsE gene of the exopolysaccharide synthesis operon of B. subtilis has been suggested to function in a manor similar to a molecular clutch#1. If expressed inside a cell it will prevent flagellar movement causing the cell to no longer be able to swim effectively and instead only tumble. As such EpsE could potentially be used as a controller of B. subtilis movement.

Though the EPS operon is normally repressed in B. subtilis, if EpsE is synthetically expressed it would be beneficial for the original copy of epsE to be knocked out. This can be achieved by integrating over the EesE gene with the epsE integration Biobricks (BBa_K143005 and BBa_K143006) which contain 2 in-frame stop codons.

Although many bacterial flaggelar assemblies contain proteins that are similar in shape, there is no guarantee that the epsE gene will function correctly in any host cell other than B. subtilis

Notes

The epsE(aka yveO) sequence was located in the B. subtilis chromosome#1 and the PstI restriction site removed before synthesis by GeneArt

Source

The epsE gene sequence was taken from the B. subtilis chromosome and was synthesised by GeneArt.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
EpsE Molecular Clutch Gene	1,834	CDS, feature/cds
start	1,3	feature/start, start_codon
stop	838,840	stop_codon, feature/stop
stop	835,837	feature/stop, stop_codon

Other Properties

• igem#experience: Works
• igem#partStatus: Released HQ 2013
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K143032/1

Member of these Collections

• //chassis/prokaryote/bsubtilis
• iGEM Parts Registry