Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Qin Qi
Date created: 2008-09-17 11:00:00
Date modified: 2015-05-08 01:10:24
LipA-EAK16-II Fusion Protein
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K143034_sequence (Version 1) |
Description
EAK16-II is a sixteen amino acid peptide that self-assembles to form β-sheet structures in an aqueous medium. The alternating positive and negative charges (--++--++) are responsible for creating an electrostatic attraction between adjacent peptides 1, triggering self-assembly when the EAK16-II peptides are exposed to physiological media or salt solution. When examined under SEM, a well-ordered nanofibre structure is formed by the association of the EAK16-II peptides and these nanofibres can futher aggregate to form a membranous 3D scaffold.LipA is a signal peptide from the B.subtilis genome. In general, signal peptides are responsible for directing preproteins (secretory proteins with a signal peptide region attached)through an appropriate secretory pathway2. LipA has been successfully used in the secretion of heterologous proteins such as cutinase by B. subtilis.