XylR

BBa_K143036 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K143036

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Chris Hirst

Date created: 2008-09-15 11:00:00

Date modified: 2015-05-08 01:10:24

Xylose operon regulatory protein

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K143036_sequence (Version 1)

Description

Transcription is regulated by proteins which bind operator sequences around the transcription start site. These proteins can positively affect transcription (activators) or negatively affect transcription (reppresors). Some repressor proteins can be inactivted however by addition of an inducer, such as xylose.

XylR if the regulator protein for the Xylose operon in B. subtilis#1 and is responsible for ensuring that in the absence of xylose the xylose metabolism proteins are not expressed. Though endogenous to B. subtilis, to minimise the leakage of a xylose inducible promoter XylR should be over-expressed. In the presence of xylose, the XylR tetramer is unable to bind DNA and so transcription resumes.

It must be noted that in all B. subtilis strains that do not have the Xylose operon knocked out the xylose inducer will gradually be metabolised by the host

XylR was used in conjunction with the Xylose operon promoter (BBa_K143014) and acted as an input adaptor for a Polymerases per second (POPS) output

Notes

The XylR protein was identified in the genome using its Genbank entry#2 and NCBI's sequence viewer and PCR primers designed from the sequence. Biobrick prefix and suffix sequences were added and the gene cloned by PCR with Pfu DNA polymerase

Source

The XylR protein was PCR cloned form the B. subtilis genome using Pfu DNA polymerase

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Xylose operon regulatory protein	1,1050	CDS, feature/cds
start	1,3	start_codon, feature/start
stop	1051,1053	feature/stop, stop_codon
stop	1054,1056	stop_codon, feature/stop

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K143036/1

Member of these Collections

• //chassis/prokaryote/bsubtilis
• iGEM Parts Registry