Pctc-gsiB

BBa_K143058 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K143058
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chris Hirst
Date created: 2008-10-05 11:00:00
Date modified: 2015-05-08 01:10:24

Promoter ctc and RBS gsiB for B. subtilis



Types
DnaRegion

Roles
Generator

engineered_region

Sequences BBa_K143058_sequence (Version 1)

Description

Sigma B dependant promoter ctc(BBa_K143010) coupled to the strong Ribosome Binding Site gsiB(BBa_K143020) from B. subtilis.

In B. subtilis endogenous sigma factor B is activated under mild stress. These mild stress conditions can be generally split into nutrient stress response and physical stress response. Nutrient stress response is triggered by low levels of ATP and GTP and physical stress response is triggered by exposure to blue light, salt, heat, acid or ethanol1. The promoter ctc has been used previously as a read out for the activation of sigma factor B 2.

Pctc has been used to take an input of blue light and give a Ribosomes per second(RiPS) output.
To work as a blue light receiver correctly, over-expression of the blue light receptor YtvA (BBa_K143037) is required.

To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.

Notes

The sequence of promoter ctc was obtained from the B. subtilis genome and published papers1 2. RBS-gsiB were obtained from papers3 and the sequence synthesised by GeneArt

Source

Pctc-gsiB was synthesised by GeneArt

Access Instance Definition
public
public
BBa_K143010
BBa_K143020
Pctc
RBS-GsiB
Sequence Annotation Location Component / Role(s)
BBa_K143010
BBa_K143020
1,56
65,75
Pctc
RBS-GsiB
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K143058/1