Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chris Hirst
Date created: 2008-10-05 11:00:00
Date modified: 2015-05-08 01:10:24
Promoter gsiB and RBS spoVG for B. subtilis
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K143061_sequence (Version 1) |
Description
Sigma B dependant promoter gsiB(In B. subtilis endogenous sigma factor B is activated under mild stress. These mild stress conditions can be generally split into nutrient stress response and physical stress response. Nutrient stress response is triggered by low levels of ATP and GTP and physical stress response is triggered by exposure to blue light, salt, heat, acid or ethanol1. The promoter ctc has been used previously as a read out for the activation of sigma factor B 2.
PgsiB has been used to take an input of blue light and give a Ribosomes per second(RiPS) output. To work as a blue light receiver correctly, over-expression of the blue light receptor YtvA (
To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.
Notes
The sequence of promoter gsiB was obtained from the B. subtilis genome and published papers1 2. RBS-spoVG were obtained from papers3 and the sequence synthesised by GeneArtSource
Pctc-spoVG was synthesised by GeneArt| Access | Instance | Definition |
| public public | BBa_K143011 BBa_K143021 | PgsiB RBS-spoVG |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K143011 BBa_K143021 | 1,38 47,58 | PgsiB RBS-spoVG |