BBa_K143076

BBa_K143076 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K143076

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Chris Hirst

Date created: 2008-10-27 12:00:00

Date modified: 2015-05-08 01:10:24

B. subtilis AmyE locus RFP output promoter and RBS tester

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K143076_sequence (Version 1)

Description

Long: AmyE 3' Integration sequence(BBa_K143002) coupled to RFP proein coding region and the registry double terminator (part BBa_J04650.

The amyE 3' integration sequence allows integration into the B. subtilis genome at the amyE locus if the 5' amyE integration sequence(BBa_K143001) is cloned onto the 5' end of the construct.

The RFP coding region gives a fluorescent output when a promoter and RBS are placed immediately upstream of the coding region while the terminator prevents readthrough.

Notes

The amyE 3' integration sequence was PCR cloned from the B. subtilis integration vector pDR111 utilising Pfu DNA polymerase. RFP and the double terminator were obtained from the registry.

Source

The amyE 3' integration sequence was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick with the RFP and the double terminator that were obtained from the registry.

Components

Access	Instance	Definition
public	BBa_J04650	mRFP1
public	BBa_K143002	amyE 3 IS

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J04650	1,843	mRFP1
BBa_K143002	852,1853	amyE 3 IS

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K143076/1

Member of these Collections

• //chassis/prokaryote/bsubtilis
• iGEM Parts Registry