Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Rifei Chen, Yushan Zhang
Date created: 2014-10-09 11:00:00
Date modified: 2015-05-08 01:10:24
TRE-3G promoter+SV40 PolyA, an ideal controller of mammalian gene expression with Tet-On 3G protein
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1431301_sequence (Version 1) |
Description
pTRE-3G promoter is a kind of eukaryocyte cell promoters that will be switched on after connect with a compound which is TetOn protein and tetracycline[TetOn sequence see BBa_K1431101]. That is a very useful way to control whether protein express or not. Of course PolyA is needful in eukaryocyte cell. There are two BbsI sites between promoter and PolyA which can make the coding sequence seamless gather with them. So we can test how many nucleotides interval between promoter and coding sequence will make the highest expression efficiency.Notes
For BbsI site, we have a series same design sequence. You can get how BbsI site work by google. And the reason why we designed that because we want to make coding sequence seamless gather with promoter and PolyA or any amount nucleotides. That depend on which will make the highest efficiency. Sorry for the limited time, we only test GFP seamless gather with promoter and PolyA but did not get the data which will make the highest efficiency.Source
It is from our instructor's lab.| Sequence Annotation | Location | Component / Role(s) |
| TRE 3G promoter SV40 PolyA BbsI site BbsI site | 1,383 410,540 386,391 402,407 | feature/promoter promoter feature/polya polyA_site sequence_feature feature/misc sequence_feature feature/misc |