Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Rifei Chen, Yushan Zhang
Date created: 2014-10-09 11:00:00
Date modified: 2015-05-08 01:10:24
SV40 promoter+SV40 PolyA
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1431302_sequence (Version 1) |
Description
SV40 promoter is a kind of eukaryocyte cell promoters, of course PolyA also needful. There are two BbsI sites, which recognize six bases[GAAGAC] but cut downstream sites between two bases and four bases, between promoter and PolyA which can make the coding sequence seamless gather with them. So that we can also test how many or what kind of nucleotides interval between promoter and coding sequence will make the highest expression efficiency. For example, if we want to test the efficiency of two nucleotides interval, we should design the the primer sequence like GAAGACNN'NN***, the first NN will be any bases, the second NN is the interval bases,and *** is the begining of coding sequence. If you still do not understand, you can search how BbsI work on the internet or connect with us.Notes
For BbsI site, we have a series same design sequence. You can get how BbsI site work by google. And the reason why we designed that because we want to make coding sequence seamless gather with promoter and PolyA or any amount nucleotides. That depend on which will make the highest efficiency. Sorry for the limited time, we only test GFP seamless gather with promoter and PolyA but did not get the data which will make the highest efficiency.Source
It is from our instructor's lab.| Sequence Annotation | Location | Component / Role(s) |
| SV40 promoter SV40 PolyA BbsI site BbsI site | 5,370 401,531 377,382 393,398 | feature/promoter promoter polyA_site feature/polya feature/misc sequence_feature feature/misc sequence_feature |