BBa_K1433001

BBa_K1433001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1433001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chaofan Zhang
Date created: 2014-09-27 11:00:00
Date modified: 2015-05-08 01:10:25

attB-J23110-attP



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1433001_sequence (Version 1)

Description

Bxb1 gp35 is a serine integrase and Bxb1 gp47 is an excisionase in Mycobacterium phage Bxb1.
This part is composed of 2 elements.
1. Promoter: BBa_J23110, a middle-ground Bacterial constitutive promoter.

2. attB and attP sites: Recognition site for Bxb1 gp35, Mycobacterium Phage Bxb1 DNA integrase.
This part promotes transcription and translation downstream gene, and when treat with Bxb1 gp35, upstream gene will express.

Notes

No

Source

this parts are construct from BBa_J23110 by adding attB/attP sites using PCR

Sequence Annotation Location Component / Role(s)
attB
J23110 Promoter
attP
1,49
50,84
85,137
feature/dna sequence_feature
feature/promoter promoter
sequence_feature feature/dna
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1433001/1