Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hanqing Liu
Date created: 2014-09-27 11:00:00
Date modified: 2015-05-08 01:10:25
RBS-gp47-tag-terminator
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1433003_sequence (Version 1) |
Description
Bxb1 gp47 is an excisionase and Bxb1 gp35 is a serine integrase in Mycobacterium phage Bxb1. This intergrase along can typically catalyzes site-specific recombination and mediate DNA inversion from an BP to LR state. Heterologous expression of Bxb1 integrase gp35 and excisionase gp47 could mediate DNA restore from an LR to BP state.This part is composed of 4 elements.
1. Promoter: BBa_J23110, a middle-ground Bacterial constitutive promoter.
2. gp47: An excisionase in Mycobacterium phage Bxb1.
3. tag: A distinct proteolysis tag on gp47 peptides.
4. terminator: BBa_B0015, a strong double terminator.
This part has component encodes a distinct proteolysis tag on gp47 peptides, for fine control over gp47 synthesis and degradation.
Notes
NoSource
No| Sequence Annotation | Location | Component / Role(s) |
| RBS Bxb1 Excisonase gp47 AAK tag BBa_B0015 Terminator | 1,25 26,802 803,841 842,970 | feature/rbs ribosome_entry_site CDS feature/cds tag feature/tag stem_loop feature/stem_loop |