| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K1439004_sequence (Version 1)
|
Description
Histone H4 can efficiently mediate gene transfer, it rich in positively charged alkaline amino acids and has the ability to combine with DNA. TAT-PTD contains an 11-amino acid peptide, and these amino acids YGRKKRRQRRR are highly basic, which is crucial for penetrating the plasma membrane. We use linker to link H4 histone and TAT, thus it can seize DNA and take it into cells. Now, it becomes our transfer device.
Notes
We found the sequence from NCBI, and synthetized primer.
Source
Histone H4 comes from the Wheat histone H4 gene, and TAT-PTD comes from HIV-1's TAT protein gene.
