Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Iva Burova
Date created: 2014-10-15 11:00:00
Date modified: 2015-05-08 01:10:27
B2 3
| Types | DnaRegion |
| Roles | Other sequence_feature |
| Sequences | BBa_K1442115_sequence (Version 1) |
Description
Each unique RNA dependent RNA polymerase (RdRP) initiates de novo replication of a RNA strand by interacting with RdRP-specific RNA sequences, henceforth called RdRP/RNA promoters. The RdRP chosen for our project is taken from the Hepatitis C virus (HCV). The B2 RNA promoter was identified as such by Heinz, Kao (2000) , along with a number of other sequences derived from the Brome Mosaic Virus (BMV). All of them possess a few common characteristics: an initiation cytidylate at the 3??? end, where the replication starts; and a stable secondary structure ??? single stranded tail and a stem of various length. Replication stops at the 5??? end. Please refer to Section: RdRP-directed Replication for further details.This is derived from the 3??? end of the Cytomegalovirus RNA3 minus strand. It contains a non-templated guanylate at the 3??? end. These are genomic plus-strand RNAs that can direct BMV minus-strand initiation. This has a 3??? single stranded tail which is required for efficient RNA synthesis.
Notes
Gel electrophoresis showing replication by HCV RdRP of a number of potential RNA promoters. The bands show the amount of replicated product and its approximate length, and quantify the tendency promoters to produce strands of wrong size. C2 (band 8) shows considerable efficiency in directing replication of the full template, although an amount of incorrect products were also present.Source
Cytomegalovirus RNA3| Sequence Annotation | Location | Component / Role(s) |
| Ribozyme RNA Promoter | 34,105 7,33 | engineered_region feature/BioBrick promoter feature/promoter |