Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Iva Burova
Date created: 2014-10-15 11:00:00
Date modified: 2015-05-08 01:10:27
SLC8 3
| Types | DnaRegion |
| Roles | sequence_feature Other |
| Sequences | BBa_K1442116_sequence (Version 1) |
Description
Each unique RNA dependent RNA polymerase (RdRP) initiates de novo replication of a RNA strand by interacting with RdRP-specific RNA sequences, henceforth called RdRP/RNA promoters. The RdRP chosen for our project is taken from the Hepatitis C virus (HCV). The SLC8 RNA promoter was identified as such by Heinz, Kao (2000) , along with a number of other sequences. All of them possess a few common characteristics: an initiation cytidylate at the 3??? end, where the replication starts; and a stable secondary structure- single stranded tail and a stem of various length. Replication stops at the 5??? end. Please refer to Section: RdRP-directed Replication for further details.This is a genomic plus-strand RNAs that can direct BMV minus-strand initiation and is derived from the 3??? terminal of the Brome Mosaic Virus which forms tRNA like structures. There is a stem loop structure within this, named SLC, that is seen to direct replication. This is also from the BMV SLC loop but has 8 nucleotides added to it which contain the 3??? initiation site CCA.
Notes
Gel electrophoresis showing replication by HCV RdRP of a number of potential RNA promoters. The bands show the amount of replicated product and its approximate length, and quantify the tendency to produce strands of wrong size. SLC8 (lane 9) is performs very well in directing replication of the full template and produces a small amount of products with an incorrect size.Source
Brome Mosaic Virus| Sequence Annotation | Location | Component / Role(s) |
| RNA Promoter Ribozyme | 7,53 54,123 | promoter feature/promoter engineered_region feature/BioBrick |