Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Iva Burova
Date created: 2014-10-15 11:00:00
Date modified: 2015-05-08 01:10:27
Sldel+8 3
| Types | DnaRegion |
| Roles | sequence_feature Other |
| Sequences | BBa_K1442117_sequence (Version 1) |
Description
Each unique RNA dependent RNA polymerase (RdRP) initiates de novo replication of a RNA strand by interacting with RdRP-specific RNA sequences, henceforth called RdRP/RNA promoters. The RdRP chosen for our project is taken from the Hepatitis C virus (HCV). The SLdel+8 RNA promoter was identified as such by Heinz, Kao (2000) , along with a number of other sequences. All of them possess a few common characteristics: an initiation cytidylate at the 3??? end, where the replication starts; and a stable secondary structure- single stranded tail and a stem of various length. Replication stops at the 5??? end. Please refer to Section: RdRP function for further details.This is a genomic plus-strand RNA that can direct BMV minus-strand initiation. This has 8 nucleotides at the 5???end deleted and shows that the 5??? end is not essential for NS5B action.
Notes
Gel electrophoresis showing replication by HCV RdRP of a number of potential RNA promoters. The bands show the amount of replicated product and its approximate length, and quantify the tendency to produce strands of wrong size. SLdel+8 (lane 10) does not perform very well in directing replication of a correct sized template and produces a large amount of faulty products.Source
Brome Mosaic Virus| Sequence Annotation | Location | Component / Role(s) |
| RNA Promoter Ribozyme | 7,44 45,114 | feature/promoter promoter feature/BioBrick engineered_region |