BBa_K1444001

BBa_K1444001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1444001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Dan Ziemianowicz

Date created: 2014-10-07 11:00:00

Date modified: 2015-05-08 01:10:27

Composite promoter and weak B. subtilis RBS - pLacI x3

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K1444001_sequence (Version 1)

Description

A composite part consisting of the constitutive promoter Pveg (BBa_K823003), followed by three copies of the repressible promoter pLacI (BBa_R0010), and ending with the weak RBS from B. subtilis (BBa_K780002).

This composite part was intended to control the expression of a downstream reporter, regulated by the expression of the pLacI repressor protein. The constitutive promoter Pveg allows reliable expression when the repressible promoter is not repressed. The triplicate repressible promoter allows for stronger repression.

Notes

No interference with endogenous cell signalling.

Source

B. subtilis

Components

Access	Instance	Definition
public	BBa_K823003	BBa_K823003
public	BBa_R0010	LacI
public	BBa_R0010	LacI
public	BBa_R0010	LacI
public	BBa_K780002	R37

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K823003	1,237	BBa_K823003
BBa_R0010	246,445	LacI
BBa_R0010	454,653	LacI
BBa_R0010	662,861	LacI
BBa_K780002	870,885	R37

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1444001/1

Member of these Collections

• iGEM Parts Registry