BBa_K1444025

BBa_K1444025 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1444025

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Dan Ziemianowicz

Date created: 2014-10-16 11:00:00

Date modified: 2015-05-08 01:10:27

Composite promoter and weak B.subtilis RBS - C2-P22 x3

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K1444025_sequence (Version 1)

Description

A composite part consisting of the constitutive promoter Pveg BBa_K823003, followed by three copies of the repressible promoter C2-P22 BBa_R0053, and ending with the weak RBS from B. subtilis BBa_K780002.

This composite part was intended to control the expression of a downstream reporter, regulated by the expression of the C2-P22 repressor protein. The constitutive promoter Pveg allows reliable expression when the repressible promoter is not repressed. The triplicate repressible promoter allows for stronger repression.

Notes

No interference with endogenous cell signalling.

Source

B. subtilis

Components

Access	Instance	Definition
public	BBa_K823003	BBa_K823003
public	BBa_R0053	cII p22
public	BBa_R0053	cII p22
public	BBa_R0053	cII p22
public	BBa_K780002	R37

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K823003	1,237	BBa_K823003
BBa_R0053	246,299	cII p22
BBa_R0053	308,361	cII p22
BBa_R0053	370,423	cII p22
BBa_K780002	432,447	R37

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1444025/1

Member of these Collections

• iGEM Parts Registry